Toxigenic Aspergillus species produce mycotoxins that are carcinogenic, hepatotoxic and teratogenic immunosuppressing agents in both human and animals. Kenya frequently experiences outbreaks of aflatoxicosis with the worst occurring in 2004-2010, which resulted in 125 deaths. This study sought to find possible reasons for frequent aflatoxicosis outbreaks in Kenya by isolating Aspergillus flavus strains from maize kernels sampled from different climatic regions of Kenya. Using diagonal transect random sampling, maize kernels were collected from Makueni, Homa Bay, Nandi, and Kisumu regions. The genetic diversity and variation among the isolates was examined by characterising the strains according to morphology, phenotype, VCGs and molecular systematics. Selected atoxigenic and aflatoxigenic A. flavus isolates were analysed using qPCR and bioanalytical techniques. The maize lines grown in various regions were also examined as a basis for an in vitro biocontrol assay. This is the first reported study in Nandi, Homa Bay and Kisumu regions. The findings could assist researchers in the selection of biocontrol strategies to mitigate aflatoxin contamination.
Alfred Mitema, is an aspiring young researcher and a lecturer in the School of Biological Sciences at the University of Nairobi in Kenya. He earned his PhD in Molecular and Cell Biology at the University of Cape Town in South Africa. He is currently a Postdoctoral Fellow at Vaal University of Technology in South Africa. He has published his work.
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LAP LAMBERT Academic Publishing
aflatoxins, Morphotype, Genetic Diversity, Heterokaryon compatibility, TLC, HPLC, Fluorescence
SCIENCE / Biology