978-620-0-31181-8

Interferon LAMBDA-3: Immune Modulator

Role of pcDNA-Interferon LAMBDA-3 (pcDNA-IFNL3) as Immune Modulator

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Summary:

Interferon Lambdas (IFNLs) is a type III IFN which induces expression of Interferon Stimulated Genes (ISGs). These ISGs in turn participate in antiviral and other interferon related activities. IFNLs are found to have immune modulation activities in addition to antiviral activities. Immune modulation activities are advantageous for the host system for better protection against invading pathogens. In the present study, the vector construct of bovine Interferon lambda-3 (IFN-L3) was analyzed for its immune modulation activities. The IFNL3 gene of 585bp cloned in pcDNA3.1+ vector (pcDNA-IFNL3) was analyzed for its expression in baby hamster kidney (BHK) cell line by fluorescence microscopy. Bovine IFNL3 gene cloned in pcDNA3.1+ vector with downstream Green fluorescent protein (GFP) tag found to show the green fluorescence. This construct was further tested for its immune modulation activities in mice. At 7th day the Spleenocytes were collected from the mice and analyzed for activation of B and T lymphocytes by Fluorescent Activated Cell Sorting (FACS) using specific CD markers. The results showed that IFNλ3 could activate both B and T lymphocyte population in treated mice.

Author:

Barathiraja S

Biographie:

I Dr. Barathiraja S, M.V.Sc., Ph.D pursued M.V.Sc from RIVER, Puducherry, India and Ph.D (Animal Biochemistry) from ICAR-IVRI, Hebbal, Bangalore, Karnataka under the mentor-ship of Dr.G.R.Reddy. At present I am working as Assistant Professor (C) in the Dept. of Veterinary Biochemistry, under the supervision of Dr.J.Thanislass, & Prof.S.Ramkumar.

Author:

Reddy G.R

Biographie:

Author:

Barathiraja S

Biographie:

I Dr. Barathiraja S, M.V.Sc., Ph.D pursued M.V.Sc from RIVER, Puducherry, India and Ph.D (Animal Biochemistry) from ICAR-IVRI, Hebbal, Bangalore, Karnataka under the mentor-ship of Dr.G.R.Reddy. At present I am working as Assistant Professor (C) in the Dept. of Veterinary Biochemistry, under the supervision of Dr.J.Thanislass, & Prof.S.Ramkumar.

Number of Pages:

56

Book language:

English

Published On:

2019-09-16

ISBN:

978-620-0-31181-8

Publishing House:

LAP LAMBERT Academic Publishing

Keywords:

Lymphocytes activation, Bovine Interferon Lambda 3, IFNL3, fluorescence microscopy, Fluorescent Activated Cell Sorting, FACS, INTERFERON LAMBDA-3, IMMUNE MODULATOR, ROLE OF pcDNA-INTERFERON LAMBDA-3, pcDNA-IFNL3

Product category:

SCIENCE / Chemistry / General