Genomic editing not only provides the possibility of describing complex gene interactions but also has created new routes through synthetic biology. Presence of CRISPR has led to a revolution in genome editing, not only because of its suitable cost but also for its ease of use in any laboratory, regardless of its molecular biology expertise. It does not have protein engineering for any of the target genes. The system only requires a DNA construct to encode Cas9 and gRNA for the target region, and if required, a default template is also required for HDR.
Professor in molecular biology and gene engineering especially in forest trees such as poplar. Currently he is working on genome editing using CRISPR-Cas9 and -Cpf1 focused on efficient on - and off - targets.
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LAP LAMBERT Academic Publishing
CRISPR, Gene engineering
SCIENCE / Biology