Immunocytochemical reactions occur in the Crinoïd: Antedon bifida, in the Asterids: Asterina gibbosa and Asterias rubens.Various antigens (HRP or Horse-radish peroxydase, Alkalin phosphatase) were injected in the animal's coelomic cavity. Animals were "immunized". Observations were done in TEM (Transmission electronic microscopy) by the use of specific labelling and in light microscopy.IN TEM immune labelling were seen in the perinuclear space (EP) rough ergastoplasm (RE) Golgi apparatus (G) and Lysosomes (L): the"antibody" biosynthesis in Invertebrates was clearly shown though the animal kingdom. It is the first time we can observe such phenomena. We show in this book immunocytochemical reactions which were done in 2 Echinodermata (Invertebrates).
Dr. Michel Leclerc was born the 31 th January 1941, at St Benoit sur Loire (Loiret, France).He possessed a DES in Biology, A doctorat ès Sciences in Immunology, and was director of the laboratory of "Immunology of Invertebrates" at Orléans University France.
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LAP LAMBERT Academic Publishing
invertebrates, Echinodermata, ophuirids, HNF1A Gene
SCIENCE / Biology